TABLE 2.

Intracellular cytokine expression by CD3⁺ T cells following anti-SP and anti-F therapeutic antibody treatment on day 2 Post-RSV infection^a

Day	Cytokine	Total cytokine-expressing CD3+ BAL (103) ± SEMb
		nIg	anti-SP	% Reductionc	anti-F	% Reduction	anti-SP/F	% Reduction
3	IL-2	13.6 ± 3.1	15.3 ± 3.5	0	15.7 ± 1.8	0	12.5 ± 1.6	8
	IL-4	15.0 ± 3.5	14.3 ± 3.3	5	15.8 ± 1.8	0	11.8 ± 1.5	21
	IL-5	13.5 ± 3.2	14.3 ± 3.3	6	14.0 ± 1.6	0	10.1 ± 1.3	25
	IL-10	10.2 ± 2.4	11.4 ± 2.6	12	12.5 ± 1.4	0	8.4 ± 1.1	18
	IFN-γ	16.7 ± 3.9	7.6 ± 1.8	54	16.7 ± 1.9	0	13.9 ± 1.8	17
	TNF-α	16.1 ± 3.7	7.3 ± 1.7	55	16.2 ± 1.9	0	12.8 ± 3.2	21
5	IL-2	10.2 ± 0	4.4 ± 0.9	57	5.3 ± 0.6	48	2.7 ± 0.5	74
	IL-4	17.3 ± 0	3.3 ± 0.6	81	9.1 ± 1.1	53	4.3 ± 0.8	75
	IL-5	14.3 ± 0	2.6 ± 0.5	82	8.0 ± 0.9	44	3.5 ± 0.7	75
	IL-10	11.3 ± 0	1.9 ± 0.4	83	5.1 ± 0.6	55	2.3 ± 0.4	80
	IFN-γ	16.9 ± 0	4.2 ± 0.8	75	13.2 ± 1.5	22	5.4 ± 1.0	68
	TNF-α	25.0 ± 0	5.8 ± 1.1	77	13.8 ± 1.6	45	5.8 ± 1.1	77
7	IL-2	4.0 ± 0.3	3.0 ± 0.7d	25	4.8 ± 1.2	0	2.4 ± 0.3e	40
	IL-4	7.5 ± 0.6	1.6 ± 0.3f	79	4.2 ± 1.1g	44	2.6 ± 0.3h	65
	IL-5	5.0 ± 0.4	3.5 ± 0.8i	30	4.5 ± 1.1	10	2.2 ± 0.3j	56
	IL-10	2.5 ± 0.2	1.6 ± 0.3k	36	2.7 ± 0.7	0	1.6 ± 0.2l	35
	IFN-γ	11.2 ± 0.9	5.5 ± 1.2m	51	7.6 ± 2.0n	32	4.0 ± 0.5o	64
	TNF-α	10.4 ± 0.8	6.6 ± 1.4p	37	8.7 ± 2.2q	16	4.2 ± 0.5r	60

^aBALB/c mice were infected with RSV. Two days p.i., the animals were i.p. treated with 150 μg of nIg, anti-SP, anti-F, or anti-SP/F antibodies/mouse. BAL samples from three mice per group were examined on days 3, 5, and 7 p.i. The results of a representative experiment are shown.

^bData are represented as the total CD3⁺ BAL expressing IL-2, -4, -5, or -10; IFN-γ, or TNF-α per lung ± SEM. The total number of CD3⁺ BAL expressing a particular cytokine was determined by multiplying the total number of BAL by the percent CD3⁺ cells expressing that cytokine. Boldface values indicate a significant difference.

^cPercent reduction is the change in total cytokine-expressing CD3⁺ cells after anti-SP, anti-F, or anti-SP/F treatment relative to total cytokine-expressing CD3⁺ cells after nIg treatment.

^dP value comparing nIg control to anti-SP; P = 0.150.

^eP value comparing nIg control to anti-SP/F; P = 0.017.

^fP value comparing nIg control to anti-SP; P = 0.009.

^gP value comparing nIg control to anti-F; P = 0.054.

^hP value comparing nIg control to anti-SP/F; P = 0.002.

ⁱP value comparing nIg control to anti-SP; P = 0.077.

^jP value comparing nIg control to anti-SP/F; P = 0.003.

^kP value comparing nIg control to anti-SP; P = 0.017.

^lP value comparing nIg control to anti-SP/F; P = 0.031.

^mP value comparing nIg control to anti-SP; P = 0.018.

ⁿP value comparing nIg control to anti-F; P = 0.117.

^oP value comparing nIg control to anti-SP/F; P = 0.002.

^pP value comparing nIg control to anti-SP; P = 0.084.

^qP value comparing nIg control to anti-F; P = 0.110.

^rP value comparing nIg control to anti-SP/F; P = 0.003.