TABLE 2.

Primers used to introduce cleavage site mutations into FCV full-length cDNA clone

Localization	Sequencea	Amino acid change(s)	Full-length cDNA clone	Virus recovery
p5.6	1 5′-GTAAAAGAAATTTGAGACAAAGTCTCAAACTCTGAGC-3′ 37	1M→1K	pF10	−
p5.6/p32	143 5′-CCTATAAGGGCAGCCGCTTGTCCTTCTTGTGCC-3′ 175	46EA→46AA	pF22	−
p32/p39	998 5′-GGGTTTAGATCAGCCGATGTGGCAAACTCATTCTGG-3′ 1033	331ED→331AD	pF71	−
p39/p30	2056 5′-CATGGCATTTGCCGCGGAGAATGGACATAG-3′ 2085	683EAEN→683AAEN	pF65	+
p39/p30	2059 5′-GGCATTTGAAGCGGCCAATGGACATAGTGAGC-3′ 2090	683EAEN→683EAAN	pF36	−
p30/VPg	2880 5′-GGTTTAGACCCAAATCTGCCGCCAAAGGAAAAAC-3′ 2913	960EA→960AA	pF77	−
VPg/Pro-Pol	3215 5′-GTTAGCTTTGCTGAAGCCTCTGGACCCGGCACC-3′ 3247	1071ES→1071AS	pF87	−
Pro-Pol	4042 5′-CATTTCCTCAGCCACCTCTATGCTATC-3′ 4068	1345ET→1345AT	pF3	+
Pro-Pol	4262 5′-GAAAAGGTTGCTGCCGGAAAGCGAAGG-3′ 4288	1419EG→1419AG	pF5	+

^aThe sequence of the sense primer is shown. The antisense primer (not shown) was the precise reverse and complementary sequence. The nucleotide positions of the beginning and the end of each of the primers in the FCV genome are indicated. Nucleotide substitutions are in bold, and cleavage site codons are underlined.