TABLE 1.
Cell surface MHC class II expression in mock- and F-infected glial cellsa
| Cell line | Virus | 12-13 h after infection
|
16 h after infection
|
|||
|---|---|---|---|---|---|---|
| % Positive | Mean fluorescence | % Positive | Mean fluorescence | |||
| Expt 1 | His16 | Mock | 99 | 181 | 99 | 132 |
| HSV-1 (F) | 91 | 92 | 62 | 60 | ||
| His28 | Mock | 56 | 130 | 50 | 103 | |
| HSV-1(F) | 37 | 59 | 14 | 46 | ||
| Expt 2 | His16 | Mock | 96 ± 0.4 | 139 ± 9.7 | ND | ND |
| HSV-1(F) | 77 ± 3.0 | 66 ± 4.0 | ND | ND | ||
| His28 | Mock | 40 ± 2.8 | 72 ± 4.1 | ND | ND | |
| HSV-1(F) | 21 ± 0.8 | 41 ± 0.7 | ND | ND | ||
a
Cells were reacted with PE-conjugated anti-HLA-DR antibodies and subjected to FACS analyses as described in Materials and Methods. ND, not determined. In experiment 1, replicate 25-cm2 cultures were harvested, combined, and analyzed at 12 and 16 h after infection. In experiment 2, triplicate 25-cm2 flasks were harvested at 13 h after infection and standard deviation values are included.