TABLE 2.

Effect of proteosomal inhibitors on surface class II expression^a

Cell line	Virus	No treatment		MG132		Z-L3-VS
		% Positive	Mean fluorescence	% Positive	Mean fluorescence	% Positive	Mean fluorescence
Expt 1b
His16	Mock	99	264	99	299	NDd	ND
	HSV-1(F)	98	137	98	150	ND	ND
His28	Mock	50	139	51	144	ND	ND
	HSV-1(F)	36	88	35	71	ND	ND
Expt 2c
His16	Mock	83	71	89	74	88	80
	HSV-1(F)	46	48	60	54	49	45

^aCells were reacted with PE-conjugated anti-HLA-DR antibodies and subjected to FACS analyses as described in Materials and Methods.

^bCells were treated with MG132 8 h after infection and harvested for analysis at 12 (His16) or 14 (His28) h after infection.

^cCells were treated with MG132 or Z-L₃-VS 6 h postinfection and harvested for analysis 12 h after infection. The lower basal levels of surface class II in experiment 2 reflect the use of higher-passage cells in this experiment.

^dND, not determined.