FIG. 5.

DNA column elution profile of 21p and 29p. High-salt nuclear protein extract was prepared from [³⁵S]methionine-labeled VZV-infected cells and loaded (lanes L) onto a DNA-agarose gel. After extensive washing (lanes W), proteins were eluted with increasing molar concentrations of KCl. The presence of 21p and 29p in each dialyzed and concentrated fraction was determined by immunoprecipitation, SDS-PAGE, and phosphorimaging. [³⁵S]methionine-labeled protein extracts from mock (lanes M)- and VZV (lanes V)-infected cells controlled for the location of 21p and 29p. The maximum yield of 29p was obtained in the 0.6 M KCl fraction, whereas 21p was not detected in any fraction.