TABLE 1.

Oligonucleotide primers used to construct yeast bait and prey plasmids

5′ to 3′ sequencea	ORFb	REc	Locationd	Directione	Clonef
CGTCGAGCTC ATG GAA GAA CCA ATT TGT	21	SacI	30,759	Forward	21A, 21A1, 21A+B
CTCAGCGGCCGC CAT TCC AAT ACG AGC GTG AGT GAA TGG	21	NotI	31,286	Reverse	21A1
CGTCGAGCTC CCA TTC ACT CAC GCT CGT ATT GGT ATG	21	SacI	31,260	Forward	21A2
CTCAGCGGCCGC ACG GGT GTG TTC TGC TAC	21	NotI	31,826	Reverse	21A, 21A2
CGTCGAGCTC GTA GCA GAA CAC ACC CGT	21	SacI	31,809	Forward	21B
CTCAGCGGCCGC GAG AGT TGC TAA AAA AAC	21	NotI	32,909	Reverse	21B
CGTCGAGCTC GTT TTT TTA GCA ACT CTC	21	SacI	32,892	Forward	21C
CTCAGCGGCCGC TTA AGG GTC ACT CCC ACT TGT	21	NotI	33,875	Reverse	21C
CGTCGAGCTC ATG GAA ATT ACT CAG AAG	29	SacI	50,857	Forward	29A
CTCAGCGGCCGC CGC CAG GCC TAT AAA CAT	29	NotI	51,924	Reverse	29A
CGTCGAGCTC ATG TTT ATA GGC ATG GAG	29	SacI	51,907	Forward	29B
ACTCGCGGCCGC CCT ATA AGT GTC CTG CAT	29	NotI	52,581	Reverse	29B
CGTCGAGCTC ATG CAG GAC ACT TAT AGG	29	SacI	52,564	Forward	29C
CTCAGCGGCCGC GGA TTT ACT GTT TGG TGG CAT	29	NotI	53,361	Reverse	29C
CTGTCGAGCTC ATG CCA CCA AAC AGT AAA TCC	29	SacI	53,341	Forward	29D
CTCAGCGGCCGC ATT TCC CGA CGA GGG ACC	29	NotI	53,991	Reverse	29D
CGTCGAGCTC GGT CCC TCG TCG GGA AAT	29	SacI	53,974	Forward	29E
CTCAGCGGCCGC TTA AAT CAT TTC CAT TGT AAT	29	NotI	54,471	Reverse	29E

^aOligonucleotide sequences including leader (single underline) and restriction endonuclease (double underline) sites 5′ with respect to the VZV DNA sequence.

^bVZV open reading frames.

^cRestriction endonuclease (RE) sites used for cloning.

^dLocations on the VZV genome of the 5′ nucleotide.

^eDirections (5′ to 3′) with respect to ORF.

^fPCR fragment identifications (see also Fig. 6).