TABLE 2.

Comparison of the effects of select mutations in gp120_JR-CSF on the binding of MAbs 2G12 and IgG1 b12

Mutationa	Affinity of antibody for indicated mutant relative to wild type (%)
	2G12	IgG1 b12
N295A	2	80
N295Q	4	261
N332A	3	75
N339A	8	30
N339Q	91	567
N386A	21	23
N392A	4	4
N392Q	6	142
S334T	10	6
L416A	2	2
F353A	15	29
K421A	19	18
K404A	32	101
S406A	33	60
T408A	40	96
S365A	1,173	667
G458A	349	857

^aAmino acid numbering is based on the HxB2 sequence.