FIG. 3.

β-Gal expression in brain slices from mice infected during the neonatal period or as young adults. (A to C) Uncultured brain slices from mice infected with 5 × 10² PFU of RM461 within 24 h after birth (neonatal infection) and maintained for 7 (A), 14 (B), or 28 (C) days. (D) Brain slices cultured for 4 weeks from mice with neonatal infection and fed 90 days (D). (E to G) Brain slices from mice infected neonatally and maintained for 180 days. (H and I) Brain slices cultured for 4 weeks from mice infected at 6 weeks of age with 5 × 10⁴ PFU (young adult infection) and maintained for 180 days. (J and K) Brain slices cultured for 4 weeks from mice infected as young adults, but with 5 × 10³ PFU, and maintained for 180 days. (L) Brain slices cultured for 4 weeks from mice injected with MEM within 24 h after birth and maintained for 180 days.