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Expression of Tax mRNA and protein in FPM1V.EFGFP/8R cells was analyzed by RT-PCR and Western blotting. (A) Total RNAs were isolated from FPM1V.EFGFP or FPM1V.EFGFP/8R cells, and 1 μg of each RNA was subjected to RT-PCR analysis for the expression of Tax and G3PDH mRNAs. (B) Whole-cell extracts were isolated from FPM1V.EFGFP or FPM1V.EFGFP/8R cells, and 50 μg of each protein was subjected to Western blotting analysis for the expression of the Tax and α-tubulin proteins.
