FIG. 2.

Analysis of proteins encoded by VSV recombinants. BHK cells were infected with either VSV-E1₃₅₂G, VSV-E2₆₆₁G, VSV-E2₇₁₇G, VSVΔG-E1₃₅₂G, VSVΔG-E2₆₆₁G, or VSVΔG-E2₇₁₇G at an MOI of 10 and labeled with [³⁵S]methionine. (Left) Cell lysates were analyzed by SDS-10% PAGE. The positions of the five VSV proteins L (241 kDa), G (63 kDa), N (47 kDa), P (30 kDa), and M(27 kDa) are indicated. (Right) BHK cell extracts were immunoprecipitated with a rabbit anti-VSV G tail antibody and analyzed by SDS-10% PAGE. VSV M protein aggregates and appears at substantial levels in all precipitates.