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. 2026 Sep 29;18:607952. doi: 10.2147/CMAR.S607952

Figure 2.

Four Sanger trace panels with base calls; vertical red marker shows the junction point. Composite figure with four stacked Sanger sequencing chromatograms labeled e13a2, e14a2, e1a2 and e19a2. Each panel shows multicolored peak traces with the corresponding nucleotide letters printed above. A thin vertical red line marks the transition between the left segment labeled as a bcr exon (13, 14, 1, or 19) and the right segment labeled as abl exon 2, indicating the junction position in each sequence.

Electropherograms of different transcripts are analysed using Sanger sequencing; the red line indicates the location of the breakpoint of each BCR-ABL fusion transcript. e13a2, e14a2, e1a2 and e19a2 are fusions of exon 13, 14, 1 and 9 of the BCR gene, respectively, with exon 2 of the ABL gene.