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Limit of detection of the multiplex TD-PCR. Assessment of multiplex TD-PCR LoD obtained from positive control of serial dilution (1 pg-1 fg per µL) for the four transcript targets. The limit of detection of e13a2 and e14a2 is 10 fg/µL, and the limit of detection for e1a2 and e19a2 is 100 fg/µL.
Abbreviation: ntc, non-template control.
