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Fusion of Vero cells transiently expressing standard and truncated H proteins. (A) Cells were transfected with pCG-FEdm in combination with a plasmid containing standard H or truncated H genes (HEdm or HcΔ15 to HcΔ24). At 24 h posttransfection, the cells were fixed and stained with Giemsa's staining solution. Magnification, ×100. (B) Syncytium formation (% cell fusion) was quantified as described in Materials and Methods. The error bars indicate standard deviation.
