Skip to main content
. 2002 Jul;76(14):6944–6956. doi: 10.1128/JVI.76.14.6944-6956.2002

FIG. 3.

FIG. 3.

Gel mobility shift assay of HCV NS5B protein and the viral RNA. The binding assay was conducted with HCV NS5B protein and the 5BCR RNA as a probe by increasing the amounts of competitor RNAs. (A) The competitor RNAs were the unlabeled 5BCR-UTR, 5BCR, and 3′ UTR RNAs. (B) The competitor RNAs were the 5BCRa, 5BCRb, and homopolymer of poly(U) RNAs. Lanes 1, labeled 5BCR RNA as a marker for free RNA; lanes 2, probe RNA with LysN; lanes 3, probe RNA with NS5B. In the mixture of NS5B and the probe RNA, the unlabeled competitor RNAs of 1- (lanes 4, 7, and 10), 5- (lanes 5, 8, and 11), and 10-fold (lanes 6, 9, and 12) molar excess to the probe RNA were added. The products of the RNA-protein complex and free RNA are indicated at the right of each gel.