TABLE 1.
Data collection and image processing
Virus | No. of micro- graphs | Under- focus (μm)a | No. of particlesb | Mean correlation coefficientc (SD) | Resolution of the map (Å) |
---|---|---|---|---|---|
AuraT | 20 | 1.90-3.52 | 3,506 (2,812) | 0.397 (0.105) | 17.0 |
AuraB | 21 | 1.46-3.12 | 1,581 (1,263) | 0.454 (0.090) | 21.0 |
Determined from the averaged Fourier transform of boxed images.
Total number of viral particles boxed from all micrographs. The number of particles used for the three-dimensional reconstruction is given in parentheses.
Average real-space correlation coefficient for all particles computed between 165 and 350 Å radii of the final model. The correlation coefficient (CC) was calculated as follows: CC = Σ(ρ1ρ2 − <ρ1><ρ2>)/[Σ(ρ12 − <ρ1>2) Σ(ρ22 − ρ2>2)]0.5. ρ1 and ρ2 refer to the density values of one particle image and its corresponding projection of the three-dimensional reconstruction, respectively. The sum is take over all pixels within the defined radii and <ρ1> and ρ2> refer to the mean values of the image and the corresponding projection of the reconstruction. The standard deviation (SD) of each correlation coefficient is given in parentheses.