Fig. 1.

Plasmid construction and validation of protein expression. (A) Schematic diagrams of plasmids. pYL515 is the recombinant plasmid constructed in this study, carrying the FimB antigen gene; pYL590 is a laboratory-preserved plasmid containing the FimA, NetB, and Fba antigen genes.(B-C) Western blot analysis of FimB expression in L. plantarum NC8 (B), and its localization in the cell wall protein fraction (C). (D) The anchoring effect of proteins expressed by pYL515 was verified using confocal laser scanning microscopy (CLSM).(E-G) Western blot validation of the antigens FimA (E), Fba (F), and NetB (G) expressed by pYL590 in L. plantarum NC8.