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Animal study protocol and determination of antigen specific antibodies. (A) Schematic timeline of immunization, coccidiosis priming, and C.perfringens challenge. (B)Serum IgG. (C) Intestinal IgA. (D) Intestinal IgM. (E)Intestinal IgG. Data are presented as mean ± SD (n = 4 birds/group);*P < 0.05, ***P < 0.001, ****P < 0.0001 vs. PBS control group and NC8 control group.
