Skip to main content
. 2026 Sep 8;105(12):107740. doi: 10.1016/j.psj.2026.107740

Fig. 3.

Fig. 3

Detection of splenic lymphocyte proliferation and intracellular production of cytokines. (A) Splenocyte proliferation index was determined using the CCK-8 assay following in vitro stimulation with recombinant proteins (FimA, FimB, NetB, Fba) and concanavalin A (ConA) at day 14 post-immunization. (B) Relative mRNA expression levels of IL-4 and IFN-γ in splenic lymphocytes. (C) Concentrations of IL-4 and IFN-γ in splenic lymphocyte culture supernatants. (D) Relative mRNA expression levels of IL-4, IFN-γ, and CCR7 in intestinal tissues. Data are presented as mean ± SD (n = 4 per group). *P < 0.05, **P < 0.01, ***P < 0.001 vs. G1 and G2 groups.