Table 1.
Bacterial strains and plasmids.
| Materials | Description | Source |
|---|---|---|
| Bacteria strains |
||
| E.coli Top10 | E.coli host strain for regular cloning | Shenggong, China |
| E. faecalis YL-EF32 | Isolated E. faecalis strain from wild bird | Lab stock(1) |
|
L.plantarum NC8 |
Host strain, Plasmid-free, silage isolate | Thea Aukrust (1992) |
| Plasmids | ||
| pYL404 | Synthesized pgsA’-fimB fragment, codon optimized for L. plantarum | Genewiz, China |
| pYL560 | P23 promoter, prokaryotic vector in L. plantarum | Lab stock(2) |
| pYL590 | pSIP409-based prokaryotic expression vector, express PgsA-FimB,LysM-Fba,LP1261-NetB anchoring protein. | Lab stock |
| pYL515 | pSIP409-based prokaryotic expression vector (containing the PgsA’ anchoring sequence), expressing the protein FimB. | This study |