FIG. 5.

Infection of Df-1 cells by ASLV-A is inhibited by mc8C5-4. Df-1 cells were seeded onto glass coverslips in eight quadruplets. One day postseeding, cells were infected with 1,000 infectious units of RCASBP(A)-AP virus supernatant or were mock infected. Prior to infection, virions had been preincubated with seven different concentrations of mc8C5-4. Cells were cultured for 48 h before being fixed and stained for AP activity. (A) Bright-light photographs were taken of four to five random fields per each of the four coverslips per sample, at a magnification of ×20 or ×10, as indicated. (B) The blue, i.e., infected, cells per field were counted (corrected for magnification) and plotted. IC₅₀ and IC₉₀ were calculated from the resulting exponential decay curve.