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Expression of the Ub-E6 protein in vitro. Protein products of in vitro transcription-translation reactions were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Products of the pCMV-UbE6 construct are shown in lane 3. Control reactions were performed with pcDNA3 (lane 1) and the pdCMV-E6 construct (lane 2).
