TABLE 3.
Drug susceptibilities of HIV-1 protease and Gag mutant viruses determined by multiple-cycle sensitivity assay
| Virus | Mean IC50 (nM) ± SDa of:
|
|||||
|---|---|---|---|---|---|---|
| APV | IDV | LPV | NFV | RTV | SQV | |
| HXB2 | 75.7 ± 8.4 (1.0) | 33.1 ± 10.6 (1.0) | 24.4 ± 2.9 (1.0) | 21.6 ± 11.8 (1.0) | 58.5 ± 21.9 (1.0) | 7.0 ± 0.9 (1.0) |
| I54V | 39.5 ± 3.0 (0.5) | 9.4 ± 1.2 (0.4) | ||||
| I54M | 342.5 ± 38.1 (4.5) | |||||
| I54L | 217.7 ± 14.2 (2.9) | |||||
| I50V | 230.9 ± 32.2 (3.0) | 12.9 ± 0.0 (0.4) | 41.9 ± 7.8 (1.7) | 9.1 ± 2.3 (0.4) | 99.2 ± 11.9 (1.7) | 3.6 ± 1.0 (0.5) |
| L449F | 65.0 ± 21.6 (0.9) | 22.0 ± 2.3 (0.7) | 17.2 ± 1.1 (0.7) | 14.5 ± 4.0 (0.7) | 34.8 ± 4.1 (0.6) | 5.4 ± 1.6 (0.8) |
| P453L | 43.0 (0.6) | |||||
| I50V M46I | 816.1 ± 66.2 (10.8) | 190.4 ± 56.3 (6.4) | ||||
| I50V L449F | 772.5 ± 104.1 (10.2) | 22.0 ± 0.8 (0.7) | 84.4 ± 6.3 (3.5) | 16.1 ± 8.0 (0.7) | 157.4 ± 3.3 (2.7) | 4.6 ± 0.9 (0.7) |
| I50V P453L | 815.0 ± 106.5 (10.8) | 43.8 ± 21.4 (1.3) | 116.8 ± 24.0 (4.8) | 28.5 ± 2.1 (1.3) | 216.2 ± 12.7 (3.7) | 6.7 ± 1.7 (1.0) |
| I50V M46I L449F | 1,150.9 ± 41.5 (15.2) | 59.2 ± 21.9 (1.8) | 189.7 ± 24.1 (7.8) | 81.4 ± 45.4 (3.8) | 396.8 ± 149.7 (6.8) | 7.2 ± 0.6 (1.0) |
| I50V M46I P453L | 1,203.2 ± 70.0 (15.9) | 53.4 ± 24.3 (1.6) | 189.5 ± 14.9 (7.8) | 71.6 ± 42.1 (3.3) | 402.6 ± 179.8 (6.9) | 7.3 ± 1.4 (1.0) |
a
All experiments were done in triplicate. All APV IC50s for I50V-containing mutants are the results of five or more independent experiments. Numbers in Parentheses are fold shifts in sensitivities relative to that for HXB2. NFV, nelfinavir; SQV, saquinavir.