TABLE 5.
Catalytic efficiencies of HIV-1 wt and I50V proteases with wt and p1/p6 mutant peptide substrates
| Peptide | Sequence |
kcat/Km (μM−1 s−1) for:
|
|
|---|---|---|---|
| E(wt) | E(I50V) | ||
| MA/CA | Dabcyl-γAbu VSQNY/PIVQNγAbu-Edans | 0.162 ± 0.005 | 0.0028 ± 0.0001 |
| CA/p2 | Dabcyl-γAbu KARVL/AEAMSγAbu-Edans | 0.27 ± 0.01 | 0.0130 ± 0.0004 |
| P2/NC | Dabcyl-γAbuSATIM/MORGNγAbu-Edans | 0.53 ± 0.01 | 0.0070 ± 0.0001 |
| NC/p1 | Dabcyl-γAbuERQAN/FLGKIγAbu-Edans | 0.00051 ± 0.00001 | 0.00002 ± 0.00001 |
| NC/TFP | Dabcyl-γAbuERQAN/FLREDγAbu-Edans | 0.00010 ± 0.00001 | <0.00001 |
| TFP/P6pol | Dabcyl-γAbuEDLAF/LQGKAγAbu-Edans | 0.0022 ± 0.0001 | 0.000060 ± 0.000002 |
| P1/p6gag | Dabcyl-γAbuRPGNF/LQSRPγAbu-Edans | 0.0198 ± 0.0001 | 0.00006 ± 0.00001 |
| Dabcyl-γAbuRPGNF/LQSRLγAbu-Edans (P453L) | 0.0620 ± 0.0006 | 0.00061 ± 0.00002 | |
| Dabcyl-γAbuRPGNF/FQSRPγAbu-Edans (L449F) | 0.1660 ± 0.0030 | 0.00139 ± 0.00002 | |
| P6pol/PR | Dabcyl-γAbuVSFNF/PQVTLγAbu-Edans | 0.115 ± 0.004 | 0.0049 ± 0.0001 |
| PR/RT | Dabcyl-γAbuCTLNF/PISPIγAbu-Edans | 0.028 ± 0.001 | 0.00067 ± 0.00002 |
| p51/p66 | Dabcyl-γAbuGAETF/YVDGAγAbu-Edans | 0.150 ± 0.002 | 0.0078 ± 0.0001 |
| p66/INT | Dabcyl-γAbuIRKVL/FLDGIγAbu-Edans | 0.235 ± 0.008 | 0.0101 ± 0.0003 |
| NEF | Dabcyl-γAbuAACAW/LEAQEγAbu-Edans | 0.076 ± 0.001 | 0.0024 ± 0.0001 |