Table 1.

Rate constants for cleavage by HDV ribozymes under single-turnover conditions

ribozyme	conditions*	rate /min−1	SD	number of replicates
Ackermannviridae ribozyme
J1/2 open		N/A
P4 open		4.6	0.40	5
P4 open C58U		N/A
C. briggsae ribozyme
J1/2 open WT		0.044	0.0016	5
P4 open WT		9.3	1.01	4
P4 open WT	2 mM Ca2+	11.1	1.45	4
P4 open WT	2 mM Mn2+	6.7	1.59	4
P4 open WT	MES (pH 6.0)	2.4	0.29	4
P4 open WT	MES (pH 6.7)	5.1	0.50	3
P4 open WT	Tris (pH 8.5)	9.9	1.12	4
P4 open C57U		N/A
P4 open G25O6S		0.010	0.0016	3

* other than standard conditions 20 mM Tris.HCl (pH 7.5), 2 mM MgCl₂.

N/A no activity detectable; SD standard deviation.