Table 1.

Primers used to detect disinfectant resistance genes.

Gene	Forward primer (5′–3′)	Reverse primer (5′–3′)	Product (bp)	Annealing temperature (°C)
aceI	CGGGCATTAATCTCGATCTGTACA	GCAGCTCACAGACCACAACAATAGT	966	58
emrE	ATGACCAACTATCTCTACCTCGC	TCAGTGCACCGAAGCGCGGA	333	55
merA	GACCACGCCGAGTTGCGTCTA	GCAGCAAGAAAGCTGCTTCA	462	62
qacEΔ1-sul1	TAGCGAGGGCTTTACTAAGC	ATTCAGAATGCCGAACACCG	300	57
tehA	ATGCAGAGCGATAAAGTGCTC	TGGACTGTATCTGCCGACAG	436	55
oqxA	GCGCTCGGCGCGATGATGCTC	CTGCTCCCACTCTTCACGGGA	402	55

All primer sequences were re-verified against the primary sources cited and confirmed in silico by NCBI Primer-BLAST against the corresponding reference sequences; predicted amplicon lengths correspond to those listed.