FIG. 1.
Efficiency of the Gag-specific cytotoxic T-cell response after DNA coinjection. Mice were immunized with 10 (A) or 100 (B) μg of pCMV.ΔR8-2 + pCMV.AS (open diamond) or pCMV.ΔR8-2 + pCMV.VSV (solid squares) plasmid DNA encoding naked or VSV-G-pseudotyped Gag particles, respectively. (C) Mice were immunized with 100 μg of pCMV.ΔR8-2 + pCMV.VSV. DNA was injected into normal muscle. Cytotoxic activity of in vitro-stimulated spleen T cells was measured 2 weeks after immunization. The specific lysis was calculated by subtracting the nonspecific lysis on P815 target cells from the specific lysis obtained on P815 cells pulsed with HIV-1 p24gag peptide (A and B). (C) Target cells were infected with either wild-type vaccinia virus (open triangles) or recombinant vaccinia virus expressing the HIV-1 Gag protein (solid circles). Specific lysis values represent mean values ± standard errors from three to five individual mice in each immunization group. E:T ratio, effector/target ratio.