FIG. 4.
Quantitation of Env-specific memory CTL after priming with VSV-Env and boosting with rVV. BALB/c mice (n = 2) were vaccinated with 5 × 106 PFU of VSV-Env and were boosted 120 days later with 5 × 106 PFU of vPE16. Bulk splenocytes were isolated 150 days after the boosting and were stained with MHC-I Env tetramers, anti-CD8 antibodies, and antibodies to CD62L (upper plots) or CD44 (lower plots) and analyzed by flow cytometry. Only CD8+ cells were included in the analysis. The results for both mice are shown.