FIG. 9.
Quantitation of Gag-specific memory CTL after priming with VSV-Env and boosting with rVV or glycoprotein exchange VSV-Gag. BALB/c mice were vaccinated with 5 × 106 PFU of VSV-Env and boosted 30 days later with 5 × 106 PFU of vvK1 (A) or 5 × 106 PFU of VSVCh-Gag (B) (n = 2 per prime-boost condition). Bulk splenocytes were isolated 30 days after boosting and stained with MHC-I Gag tetramers, anti-CD8 antibodies, and antibodies to CD62L (left plots) or CD44 (right plots) and analyzed by flow cytometry. Only CD8+ cells were included in the analysis.