FIG. 2.

Infectivity of full-length HAV RNA transcripts with deletions in 2A. (A) For each genome-length RNA transcript, the extent of the deletion is indicated, both by the positions within the polyprotein of the corresponding deleted amino acids and by the number of amino acid residues deleted. The infectivity of deleted transcripts is indicated (+, infectious; −, lethal), as determined by RIFA in BS-C-1 cells inoculated with FRhK-4 cell lysates harvested 2 weeks posttransfection of RNA. (B) RIFA autoradiograms for selected progeny viruses.