FIG. 5.

Single-cycle growth and RNA replication kinetics of vΔ2A-11 variant. FRhK-4 cells were infected at an MOI of 4 RFU/cell, either with the virus carrying the most-extensive deletion (vΔ2A-11 [open circles]) or with the parent virus (v18f [closed circles]). (A) Cytoplasmic RNAs were harvested at the indicated times p.i., denatured, immobilized onto a nylon membrane, and hybridized to an HAV-specific, ³²P-labeled, negative-strand riboprobe. HAV-specific positive-strand RNA was quantified by PhosphorImager analysis. Results of one experiment are represented and expressed as the relative percentage of the maximum hybridization signal obtained in the experiment. (B) Virus present in the supernatant and within the cells was harvested at the indicated times p.i. and titrated by RIFA. Each point represents the mean of three independent experiments. Error bars indicate the standard deviation.