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. 2002 Aug;76(15):7495–7505. doi: 10.1128/JVI.76.15.7495-7505.2002

FIG. 8.

FIG. 8.

Effect of N-terminal 2A deletions on assembly of capsid proteins expressed by recombinant VVs. FRhK-4 cells were coinfected with vTF7-3 and virus expressing either the parent polyprotein, VV-18f (A), or the indicated mutant polyproteins, VV-Δ2A-5 (B) or VV-Δ2A-3 (C), each at an MOI of 5 PFU/cell. Morphogenesis intermediates were fractionated on 5 to 45% sucrose gradients and loaded on an SDS-3.5 M urea-12% polyacrylamide gel, and their polypeptide composition was determined by immunoblot analysis using a mixture of anti-VP1 and anti-VP2 antibodies. All fractions are shown for the gradient containing the parent VV-18f (A), whereas only fractions in which pentamers (fraction 14 [P]) and empty capsids (fraction 8 [EC]) were expected are shown for the mutant viruses (B and C).