Table 3.
Calibration data and contents of compounds 1–6 in FLH and SLH extracts.
| Compound | Calibration data |
Content (mg/g extract) |
||||||
|---|---|---|---|---|---|---|---|---|
| tR (min) a | Range (μg/mL) | Calibration equation | R2 b | LOD c (μg/mL) |
LOQ d (μg/mL) |
FLH | SLH | |
| 1 | 32.85 | 15.63–250 | y = 26,033× + 145,221 | 0.9998 | 4.095 | 12.412 | 12.89 ± 0.11 | 10.37 ± 0.15 |
| 2 | 35.47 | 15.63–250 | y = 33,097× - 33,509 | 0.9996 | 3.308 | 10.025 | 1.29 ± 0.03 | 1.08 ± 0.05 |
| 3 | 35.82 | 15.63–250 | y = 12,799× + 8457.7 | 0.9997 | 1.463 | 4.432 | 0.79 ± 0.01 | 1.18 ± 0.01 |
| 4 | 38.74 | 15.63–250 | y = 28,840× + 156,255 | 0.9998 | 2.658 | 7.782 | 0.21 ± 0.01 | 0.54 ± 0.15 |
| 5 | 43.47 | 15.63–250 | y = 12,506× + 74,631 | 0.9997 | 1.013 | 3.068 | 4.28 ± 0.17 | 9.19 ± 0.22 |
| 6 | 49.81 | 15.63–250 | y = 30,451× + 7964 | 0.9998 | 7.690 | 23.302 | 0.43 ± 0.02 | 0.08 ± 0.02 |
| Total | 19.89 | 22.44 | ||||||
a Retention time; b Coefficient of determination; c Limit of detection; d Limit of quantification. Compounds: kaempferol 3,7-di-O-rhamnoside (1), kaempferol 7-O-neohesperidoside (2), kaempferol 3-O-glucoside (3), kaempferol 3-O-rhamnoside (4), kaempferol 7-O-rhamnoside (5), and kaempferol (6).