FIG. 3.

Sucrose gradient analysis of scaffolds. Scaffold particles purified from AcUL26.5 (a and b)- or AcVP22a (c and d)-infected Sf21 cells were analyzed on 5-ml, 10 to 40% (wt/vol) sucrose gradients at pH 7.2 (a and c) or pH 5.5 (b and d). Centrifugation was at 40,000 rpm for 105 min in a Sorvall AH650 rotor. Nine fractions were collected from the bottom of the gradient, and 20-μl samples of each fraction were analyzed on SDS-10% polyacrylamide gels and transferred to nitrocellulose membranes. To reveal the distribution of the scaffolding proteins, the blots were probed with MCA406 antibody as described in Materials and Methods.