TABLE 2.
Sequences of individual clones from the PBS-Pro rTΨC libraryb
| AACTTGGGCa | Shift | Predicted size of bp region | U5 RNA nucleotides |
|---|---|---|---|
| TAGGACCCGb | −1 | 7 | 71-77 |
| ACGAGCCTG | +2 | 7 | 74-80 |
| CCGGGCCTG | +2 | 7 | 74-80 |
| TAGAACCCA | −1 | 6 | 71-76 |
| TAGAACCCA | −1 | 6 | 71-76 |
| CGGAACCCT | +1 | 6 | 73-78 |
| AAGAACCCT | +1 | 6 | 73-78 |
| ACGGACCCT | +1 | 6 | 73-78 |
| CGGGACCCT | +1 | 6 | 73-78 |
| AAGAACTCC | 0 | 6 | 72-77 |
| CTGGACTGC | +3 | 6 | 74-79 |
| GAGCACCTG | +2 | 6 of 7 | 74-80 |
| ACGAGCTGC | +3 | 5 | 75-79 |
| ACGAGCTGC | +3 | 5 | 75-79 |
| CGGAGCTGC | +3 | 5 | 75-79 |
| ACGAGCTGC | +3 | 5 | 75-79 |
| CGGGACTGC | +3 | 5 | 75-79 |
| CGGGACTGC | +3 | 5 | 75-79 |
| CCGGACTGC | +3 | 5 | 75-79 |
| GCGGGCTGC | +3 | 5 | 75-79 |
| TCGGGCTGC | +3 | 5 | 74-78c |
| GATAACCCT | +1 | 5 | 74-78 |
| ACGAACACC | 0 | 5 of 6 | 72-77 |
| GACAGCTGC | +3 | 4 | 76-79 |
a
The sequence of the TΨC loop of the tRNAPro primer that can base pair with U5 RNA and which is presented as deoxynucleotides for sequence comparison. Nucleotides differing from the TΨC loop of the tRNATrp are shown in boldface type.
b
Notations are explained in the legend to Table 1.
c
Numbering 74-78 instead of 75-79 is because one of the upstream randomized bases was deleted in this clone.