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Structure of the HCV subgenomic replicon, I377/NS3-3′. The structure of the replicon is composed of the HCV 5′-NCR (solid line), the neo gene (open box), encephalomyocarditis virus IRES (solid line), coding region of NS3 to NS5B (open box), and the HCV 3′-NCR. The neomycin resistance marker allows the establishment of a transformed cell line that stably expresses replicons in RNA form.
