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. 2002 Aug;76(15):7453–7459. doi: 10.1128/JVI.76.15.7453-7459.2002

FIG. 2.

FIG. 2.

GRP78 gene expression is stimulated by HCV replicons and HCV nonstructural proteins. (A) HCV replicons and HCV nonstructural proteins activate GRP78 transcription in an ERSE-dependent manner. Huh7 and FCA4 cells were transfected with GRP78 wild-type (left panel) or mutant (right panel) luciferase reporter plasmids (white). Huh7 cells were also cotransfected with pCMV/729-3010 or pCMV and wild-type (left panel) or mutant (right panel) GRP78-Luc (black). The GRP78 luciferase reporter has three ERSE sequences. The mutant GRP78-Luc has all three of these ERSE sequences eliminated. (B) RNase protection assay indicating an increase in GRP78 mRNA steady-state levels in FCA4 cells. RNA was extracted from Huh7 and FCA4 cells and analyzed using a probe specific to GRP78. Lane 1, probe alone; lane 2, Huh7 GRP78 mRNA analysis; lane 3, FCA4 GRP78 mRNA analysis.