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. 2002 Aug;76(15):7453–7459. doi: 10.1128/JVI.76.15.7453-7459.2002

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Copyright © 2002, American Society for Microbiology

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FIG. 4. — Effects of HCV replicons or HCV nonstructural proteins on eIF-2α phosphorylation levels in cells with or without PKR. (A) FCA4 cells with HCV replicons have lower levels of eIF-2α phosphorylation. Top panel, Huh7 and FCA4 cell lysates were analyzed by Western blot analysis using anti-eIF-2α serum specific to phosphoserine-51. Bottom panel, Western blot analysis of Huh7 and FCA4 cell lysates with antiserum reactive with all forms of eIF-2α. (B) NS5A stimulates eIF-2α phosphorylation in PKR knockout cells (PKR^0/0). Western blot analysis of eIF-2α phosphoprotein from PKR^0/0 transfected with pCMV (lane 1), pCNS5A (lane 2), and pCMV/3010 (lane 3) expression vectors.