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. 2002 Aug;76(15):7453–7459. doi: 10.1128/JVI.76.15.7453-7459.2002

FIG. 5.

FIG. 5.

HCV IRES-directed translation and cap-dependent translation increases in cells with HCV replicons. (A) HCV replicons induce HCV IRES translation and cap-dependent translation. Huh7 and FCA4 cells were transfected with a dual luciferase reporter plasmid containing the HCV IRES linked to Renilla luciferase (left panel). Cap-dependent translation activity was measured in Huh7 and FCA4 cells from firefly luciferase activity using the same dual luciferase reporter plasmid (right panel). (B) Wild-type HCV replicons enhance HCV IRES activity more than replication defective HCV replicons. Huh7 cells were transfected with an HCV IRES RNA luciferase reporter alone, T7C1-341, and a wild-type HCV replicon (BB7) or a replication-defective mutant HCV replicon (BB7 pol−) was transfected into Huh7 cells along with the luciferase reporter, T7C1-341.