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. 2002 Aug;76(15):7453–7459. doi: 10.1128/JVI.76.15.7453-7459.2002

FIG. 6.

FIG. 6.

HCV replicons induce GRP78 translation while lowering overall levels of GRP78 protein. (A) HCV replicons and HCV nonstructural proteins activate GRP78 IRES-mediated translation. Huh7 and FCA4 cells were transfected with an GRP78 IRES-linked luciferase reporter plasmid (white). Huh7 cells were also cotransfected with GRP78 IRES-Luc and pCMV/729-3019 or pCMV (black). (B) GRP78 protein levels in Huh7 and FCA4 cells. Shown is a Western blot analysis of Huh7 and FCA4 cell lysates with anti-GRP78 antibody. Lanes 1 and 2, 1 μg of Huh7 and FCA4 cell lysates, respectively; lanes 3 and 4, 5 μg of Huh7 and FCA4 cell lysates, respectively.