FIG. 3.

VP1- and VP2-specific CTL assay. VP1⁺ transgenic and littermate nontransgenic mice were infected intracerebrally with TMEV and CNS-ILs harvested after 7 days for the CTL assay. The CNS-ILs were incubated with ⁵¹Cr-labeled C57SV cells transfected with either the VP1 or VP2 transgene, and lysis was determined by the level of chromium release. (A) CNS-ILs from TMEV-infected VP1⁺ transgenic mice did not lyse VP1-transfected C57SV cells, whereas those from nontransgenic littermates did. (B) In contrast, CNS-ILs from both VP1 transgenic and VP1 nontransgenic littermate mice infected with TMEV lysed VP2-transfected cells. Data shown are means of triplicate samples using pooled lymphocytes from the CNSs of four or five mice per experimental group.