FIG. 5.

Detection of VP1- and VP2-specific IgG in transgenic mice infected with TMEV. LP⁺ transgenic, VP1⁺ transgenic, and littermate control nontransgenic mice were infected intracerebrally with 2 × 10⁶ PFU of TMEV, and sera were collected 45 days later for antibody analysis. VP1- and VP2-specifc IgG levels were determined by ELISA (A and C) or Western blotting (B and D) using recombinant virus capsid antigens. Diluted sera from individual mice were added to the coated plates or blots, and bound IgGs were detected using an alkaline phosphatase system. TMEV-infected VP1⁺ transgenic mice generated antibodies against the VP1 protein (A and B). Similarly, TMEV-infected LP⁺ transgenic mice generated antibodies against the VP2 protein, which is encompassed within the LP region of the genome (C and D).