FIG. 8.
Detection of TMEV RNA persistence in spinal cord white matter of TMEV transgenic mice. Using paraffin, spinal cord sections were prepared from mice intracerebrally infected with TMEV for 45 days. TMEV RNA was localized by hybridization with a 35S-labeled probe corresponding to the VP1 region of TMEV. Viral RNA was detected in VP1+ (A), LP+ (B), and RP+ (C) transgenic mice but not in VP1− (D), LP− (E), and RP− (F) nontransgenic littermate controls. Sections were counterstained with Mayer's hematoxylin. Magnification, ×600.