Fig. 4.

(A) Growth curves (weight vs. age) of control mice, Lamb2−/− mice, and Lamb2−/− mice carrying transgenes. Lamb2−/− and Lamb2−/−; NEPH-B2 mice fail to surpass a weight of ~4 g and die at 3 to 4 weeks of age. In contrast, Lamb2−/−; MCK-B2 mice grow at a rate similar to controls but die at 1 month of age. Lamb2−/−; MCK-B2; NEPH-B2 doubly transgenic mice exhibit normal weight gain and a long life. (B) Proteinuria in Lamb2−/−; MCK-B2 mice. One μL of urine was analyzed by SDS-PAGE and stained with Coomassie blue. M, markers; the pink band is albumin. Lanes 1, 5, and 6: urine from Lamb2+/−; MCK-B2 mice. Lanes 2, 4, and 7: urine from Lamb2−/−; MCK-B2 mice. Lane 3 was empty.