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. Author manuscript; available in PMC: 2006 Oct 24.
Published in final edited form as: FEBS Lett. 2005 Oct 24;579(25):5746–5750. doi: 10.1016/j.febslet.2005.09.059

Figure 2.

Figure 2

. GDI activity of KB-752 on Gαs assessed by GTPγS binding and steady-state GTPase measurements. (A) 50 nM Gαs was incubated in the absence or presence of 5 μM KB-752 for 15 minutes at room temperature prior to addition of [35S]GTPγS. Duplicate reaction aliquots were removed at indicated times and protein-bound radioactivity counted. (B) 50 nM Gαs or Gαo was incubated with the indicated concentrations of KB-752 as in (A) and GTPγS binding reactions were incubated at 20 °C for 5 minutes. (C) 100 nM Gαo or Gαs was incubated with 10 μM KB-752 for 15 minutes at room temperature prior to the addition of [γ-32P]GTP and measurement of released [32P] inorganic phosphate.