FIG. 4.

Compilation of published AAVS1-AAV ITR crossover sequences. Schematic representation of published integration sites within AAVS1 in different cell systems with respect to PCR primers used for their detection. The bottom line represents the nucleotide sequence and numbering as determined by Kotin et al. (9). The trs and RBS homology sequences, as determined by Linden et al. (13), are indicated as boxes, as is the recently described DNase-hypersensitive site (DHS-S1) (11). The primers used for detection of junction fragments are indicated below with the designations used in the respective publications. Nucleotide positions, in the 5′ to 3′ direction, are as follows: Cr2, 1222 to 1201; C, 1345 to 1326; PAAVS1, 1609 to 1593; AAVS1-2R, 2299 to 2280. Integration sites determined in the indicated studies are given as characteristic symbols above. Each symbol represents one integration site. The cell system, the primers used for determination of junction sites, and the respective references are given to the right of the symbols.