TABLE 1.
Specificity of the real-time PCR assay for detection of AAVITR-AAVS1 junctions
| Sample | Primer(s) used | Amt of DNA in PCR (μg) | Mean no. of junctions (SD) |
|---|---|---|---|
| Mock-infected cells | PAAVS1 + PITR | 1 | 0 |
| AAV-infected cells | |||
| 72 h p.i.a | PAAVS1 | 1 | 0 |
| 72 h p.i. a | PITR | 1 | 0 |
| 72 h p.i. | PAAVS1 + PITR | 1 | 5,865 ± 649 |
| Ad-2-infected cells | PAAVS1 + PITR | 1 | 0 |
| Plasmids pRVK + pTAV2-0b | PAAVS1 + PITR | —c | 0 |
a
The 72-h p.i. sample represented in Fig. 2B was analyzed with only one primer as indicated.
b
Plasmid pRVK carrying AAVS1 and plasmid pTAV2-0 carrying the wild-type AAV-2 genome were added to the PCR at 105 copies. This equals the number of HeLa genome equivalents per microgram of DNA.
c
—, 105 copies of each plasmid were used.