FIG. 1.

HIV-1-specific immune responses to overlapping 20-mer peptide pools from Gag, Pol, Env, and Nef of HIV-1; tetanus toxoid (TT); or CMV lysate as measured by the IFN-γ ELISPOT assay. (A) BMC from HIV-seronegative women. (B) BMC responses from HIV⁺ women (U.S. cohort). (C) BMC responses from HIV⁺ women (Zambian cohort). Fifty peptides each, comprising the N terminus (Pol N aa 1 to 510) or the C terminus (Pol C aa 501 to 1003) of Pol were used instead of a pool of 100 peptides (Pol aa 1 to 1003) to stimulate BMC from the Zambian volunteers. Asterisks indicate that for volunteer 1 the responses from Pol N and Pol C were added and plotted as Pol, and the same was done for Env using gp120 and gp41. Responses to CMV and TT were not measured in volunteers 4 and 5. PHA responses were >1,000 SFC/million BMC (data not shown).