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. 2002 Aug;76(15):7365–7373. doi: 10.1128/JVI.76.15.7365-7373.2002

FIG. 4.

FIG. 4.

Breast milk-derived cells stain with HLA class I tetramer reagents. HIV-1 Gag p17 (aa 17 to 28; RLRPGGKKK) HLA A∗0301-restricted tetramer staining. (A) BMC from an HLA A3+ HIV-seronegative woman. (B) BMC from volunteer 2. (C) PBMC from volunteer 2. For tetramer analysis, cells were gated by using three parameters (forward scatter versus side scatter and CD3+ T cells) in order to focus on the CD3+ T-cell population. The percentage of CD8+ T cells that stained with the tetramer is given in the upper right quadrant of each dot plot.