TABLE 3.
HIV epitope mapping of BMC from volunteer 2 (HLA haplotype, A3, A11, B35, B51)
| Peptide (aa) | aa sequencea | No. of SFC/106 cells
|
Haplotype (reference) | |
|---|---|---|---|---|
| BMC | PBMC | |||
| Pol C poolb (201-310) | 760 | 2,150 | ||
| P26 (251-270) | HPAGLKKKKSVTVLDVGDAY | 885 | ND | B35 (57) |
| P27 (261-280) | VTVLDVGDAYFSVPLDEDFR | 1,410 | ND | B35 (57) |
| P28 (271-290) | FSVPLDEDFRKYTAFTIPSI | 950 | ND | B51 (50) |
| P29 (281-300) | KYTAFTIPSINNEETPGIRYQ | 860 | ND | B51 (50) |
| Pol D poolb (301-410) | 1,375 | 2,175 | ||
| P33 (321-340) | KILEPFRKQNPDIVIYQYMD | 1,305 | ND | B35 (42, 49) |
| Nef B poolb (101-205) | 1,580 | 2,330 | ||
| N14 (131-150) | GVRYPLTFGWCYKLVPVEPD | 1,825 | ND | B35 (49) |
| N18 (171-190) | GMDDPEREVLEWRFDSRLAF | 1,595 | ND | A2 (17) |
| Gag A poolb (1-110) | 590 | 355 | ||
| G2 (11-30) | GELDRWEKIRLRPGGKKKYK | 490 | ND | A3 (14) |
| G3 (21-40) | LRPGGKKKYKLKHIVWASRE | 420 | 600 | A3 (14) |
| p17 Gag A3 (20-28) | RLRPGGKKK | ND | 620 | |
| Medium | 175 | 25 | ||
a
Published epitope for the given HLA is underlined.
b
Each peptide pool contained 10 20-mers overlapping by 10 aa, covering the indicated region of HIV protein.