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. 2006 Feb 9;34(3):928–938. doi: 10.1093/nar/gkj477

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© The Author 2006. Published by Oxford University Press. All rights reserved

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NF-Y–p63 interactions: analysis of AEC p63 mutants. (A) Structure of the p73 SAM domain: the residues corresponding to AEC mutations in p63 are indicated. (B) Immunoprecipitation analysis of the different p63 (wt and mutants) transfected in SaoS2 cells with full-length NF-YB and NF-YC. Extracts were incubated with anti-YC and control anti-thioredoxin antibodies. Western blot analysis was performed with anti-p63 (upper panel; Santa Cruz H137), anti-YB and anti-YC (lower panels) antibodies. L, load; FT, flow through; B, bound material.